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Image Search Results
Journal: Human reproduction (Oxford, England)
Article Title: A monoclonal antibody, HCL-2, raised against human luteal cells reacts with apolipoprotein-B and detects the uptake of low density lipoprotein by luteinizing granulosa cells.
doi: 10.1093/humrep/13.4.936
Figure Lengend Snippet: Figure 1. HCL-2 antigen expression in corpus luteum on day 7 detected by indirect immunofluorescence staining. (A) Haematoxylin and eosin staining. (B) Staining with HCL-2 mAb. (C) Staining with anti-apolipoprotein-B mAb (MAB012). (D) Negative control stained with anti-TNP mAb. HCL-2 antigen and apolipoprotein-B were expressed on the granular-shaped structure near the nuclei in the cytoplasm of both large (LL) and small (SL) luteal cells. Both proteins were also detected along the cell membrane, showing the similar expression profiles. Original magnification 3120. Bar 5 100 µm.
Article Snippet: The immunoglobulin isotype was determined using an isotyping kit for
Techniques: Expressing, Staining, Negative Control, Membrane
Journal: Human reproduction (Oxford, England)
Article Title: A monoclonal antibody, HCL-2, raised against human luteal cells reacts with apolipoprotein-B and detects the uptake of low density lipoprotein by luteinizing granulosa cells.
doi: 10.1093/humrep/13.4.936
Figure Lengend Snippet: Figure 5. Detection of HCL-2 antigen on human luteinizing granulosa cells cultured in a medium with or without low density lipoprotein (LDL) by indirect immunofluorescence staining. (A–D) Culture with LDL. (E–H) Culture without LDL. (A, C, E and G) Phase-contrast pictures. (B and F) HCL-2 antigen. (D and H) Negative controls (anti-TNP mAb). HCL-2 antigen was clearly detected in the cytoplasm of human granulosa cells (arrows) cultured in the medium containing LDL for 3 days (B), whereas it was hardly detected in those cultured without LDL (F). Original magnification 3240. Bar 5 50 µm.
Article Snippet: The immunoglobulin isotype was determined using an isotyping kit for
Techniques: Cell Culture, Staining